Effect of trypsin inhibitor from Crotalaria pallida
نویسندگان
چکیده
A proteinaceous trypsin inhibitor was purified from Crotalaria pallida seeds by ammonium sulfate precipitation, affinity chromatography on immobilized trypsin–Sepharose and TCA precipitation. The trypsin inhibitor, named CpaTI, had Mr of 32.5 kDa as determined by SDS-PAGE and was composed of two subunits with 27.7 and 5.6 kDa linked by disulfide bridges. CpaTI was stable at 50 °C and lost 40% of activity at 100 °C. CpaTI was also stable from pH 2 to 12 at 37 °C. CpaTI weakly inhibited chymotrypsin and elastase and its inhibition of papain, a cysteine proteinase, were indicative of its bi-functionality. CpaTI inhibited, in different degrees, digestive enzymes from Spodoptera frugiperda, Alabama argillacea, Plodia interpunctella, Anthonomus grandis and Zabrotes subfasciatus guts. In vitro and in vivo susceptibility of Callosobruchus maculatus and Ceratitis capitata to CpaTI was evaluated. C. maculatus and C. capitata enzymes were strongly susceptible, 74.4 ± 15.8% and 100.0 ± 7.3%, respectively, to CpaTI. When CpaTI was added to artificial diets and offered to both insect larvae, the results showed that C. maculatus was more susceptible to CpaTI with an LD50 of 3.0 and ED50 of 2.17%. C. capitata larvae were more resistant to CpaTI, in disagreement with the in vitro effects. The larvae were more affected at lower concentrations, causing 27% mortality and 44.4% mass decrease. The action was constant at 2–4% (w/w) with 15% mortality and 38% mass decrease. © 2006 Elsevier SAS. All rights reserved.
منابع مشابه
In-vivo study of tissue reaction to Crotalaria pallida and Sansevieria roxburghiana fibers
BACKGROUND A suture material producing least tissue reaction is considered as ideal. Other characteristics like tensile strength, capacity to sustain sterilization process enhance its acceptability. In the present situation there is a need to reascertain the relevance and utility of these materials. Among the suture materials mentioned by Sushrutacharya, Moorva (Sansevieria roxburghiana) and Sh...
متن کاملPhytochemical screening, pharmacognostic study and physicochemical evaluation of leaf of Crotalaria pallida Aiton
The present work attempts to carry out preliminary phytochemical screening, detailed pharmacognostic study and physicochemical evaluation of leaf of Crotalaria pallida. Crotalaria pallida Aiton. is a plant with vast medicinal properties. The plant has been traditionally used for the treatment of swelling of joints , fever , vermifuge, skin diseases and also The plant and its parts are reported ...
متن کاملComparative Analysis and Nutritional Assessment of Raw Seeds of Crotalaria Species
Five different species of Crotalaria were analysed for their proximate and mineral composition, protein fractions, amino acid profile of total seed proteins, fatty acid profile of seed lipids, in vitro protein digestibility vitamins (niacin and ascorbic acid) and certain anti-nutritional factors. The major findings of the study were as follows: crude protein content ranged from 12.50 27.12%, cr...
متن کاملEstrogenic and mutagenic activities of Crotalaria pallida measured by recombinant yeast assay and Ames test
BACKGROUND Crotalaria pallida Ailton is a plant belonging to the Fabaceae family, popularly known as "rattle or rattlesnake" and used in traditional medicine to treat swelling of the joints and as a vermifuge. Previous pharmacological studies have also reported anti-inflammatory, antimicrobial and antifungal activities. Nevertheless, scientific information regarding this species is scarce, and ...
متن کاملLight perception for sun-tracking is on the lamina in Crotalaria pallida (Fabaceae).
Trifoliolate leaves of Crotalaria pallida Aiton (Fabaceae) exhibit sun-tracking behavior in simulated days and in response to a fixed, oblique light. The site of light perception for sun-tracking is on the lamina, necessitating transport of a signal from the lamina to the site of response (the pulvinule at the base of the leaflet). Evidence for the site of light perception on the lamina include...
متن کامل